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ENOD-Fernanda-de-Carvalho-Niebel_WEB.jpg

Phone +33 (0) 61 28 53 24

Key words

molecular biology, reverse genetics, cell microscopy and imaging, Medicago , rhizobia, transcription factors, cell communication and signaling

Fernanda de Carvalho-Niebel

DR2 Research Director CNRS

ENOD team

RESEARCH SUBJECTS

Plant-microorganism interactions, root symbioses.

PRESENTATION

Fernanda is co-group leader of the ENOD team and in charge of the endosymbiotic infection reseach axis of the team. She started her scientific career in Ghent (Belgium) where she obtained her PhD while working in the lab of Genetics, University of Ghent, headed by Marc Van Montagu. Her PhD work focused on the study of the regulation of tobacco pathogenesis-related ß-1,3-glucanases, during which she discovered and characterized a new phenomenon of Post-Transcriptional Gene Silencing (PTGS) in plants. She joined LIPME in 1995, as an EMBO post-doc to initiate a new project aimed at understanding early signaling during the establishment of root nodule rhizobia symbiosis in legumes. She characterized new genes of Medigaco truncatula induced by rhizobia-secreted Nod factors and then as a researcher at the CNRS, conducted promoter functional studies that led to the identification of key symbiotic promoter cis-sequences and underlying key symbiotic transcription factors, which she further characterized. Her research currently combines functional genomics and microscopy-based approaches to investigate the key regulators, cellular mechanisms and bacterial cross-talk underlying rhizobia infection.

RECENT FUNDING

Live-Switch PRCI-ANR (2020-2023); CREPE-SPE-INRAE (2020-2022); FRAIB-AO-INTERUNITE-CROSS (2019-2022).

COLLABORATIONS

A. Becker (SYNMIKRO, Marburg, Germany); E. Larrainzar (University of Navarra, Pamplona, Spain); M. Marín (LMU, Munich, Germany); Peter Kaló (NAIK, Gödöllő, Hungary); F. Cartieaux, V. Hocher & S. Svistoonoff (LSTM, Montpellier); P. Frendo & E. Boncompagni (ISA, Sophia-Antipolis); R. Peyraud (iMEAN, Toulouse); N. Frei-Dit-Frey, PM. Delaux & E. Jamet (LRSV, Toulouse).

TEACHING

-Master International LABEX-TULIP (upcoming course), Master ADAM-Toulouse (tutorials), doctoral courses (La Plata Argentina, 2017).

- Supervision of Post-docs / PhD students and Masters.

COMMITTEE MEMBER

-Appointed member of the Scientific Council of the SPE-INRAE department.

-Member of the coordination committees of the national networks INRAE SYMBIPHYT (since 2021) and SYMBIFIX (2018-2020).

-Appointed member of the Scientific Council of LABEX − TULIP and scientific co-manager of the flagship project of LABEX − TULIP "Recognition and Signaling Symbiotic Reprogramming"

- Member of the recruitment committee of INRAE-BAP CRCN researchers, BAP-CRCN competition (2020).

- Member of the recruitment juries for professors or Lecturers (Nice, Montpellier, Toulouse).

- External evaluator for professor and group leader positions (UK, Denmark).

EDITORIAL ACTIVITY

-Review of manuscripts (Nature Plants, Nature Comm, Plant Cell, New Phytol…) and projects (DFG, NSF…).

SCIENTIFIC PRODUCTION

Fernanda de Carvalho-Niebel
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Phone  +33 (0) 61 28 53 27

Key words

Symbiotic nodule development, transcription factors, non-coding RNAs, transcriptional and epigenetic regulation of nodulation

Andreas Niebel

DR2 Research director CNRS

ENOD team

RESEARCH SUBJECTS

We are working on the genetic and epigenetic control of the development of symbiotic nodules in Medicago truncatula .

TRAINING

Andreas obtained his doctorate at Ghent University in 1994 in the laboratory of Marc van Montagu, studying giant feeder cells induced in plant roots by endoparasitic nematodes. As a post-doctoral fellow at EMBO, he then worked on the symbiotic legume- Rhizobium interaction at LIPM where he was then recruited as a researcher by the CNRS.

RECENT FUNDING

ANR PIOSYM (2020-2024); CNRS-LIA (International associated laboratory) France-Argentina NOCOSYM (2018-2021). MYCONODISLAND (FRAIB; 2021-2022).

COLLABORATIONS

International: F. Ariel (Instituto de Agrobiotecnología del Litoral (IAL), Santa Fe, Argentina); F. Blanco & E. Zanetti (Instituto de biotechnologia y biologia molecular (IBBM), La Plata, Argentina); E. Larrainzar (Public University of Navarra (UPNA), Spain; K. Szczyglowski (University of Western Ontario, Canada); Senjuti Sinharoy (National Institute of Plant Genome Research, New Delhi, India). National: M. Benhamed, M. Crespi, F. Frugier (IPS2, Paris-Saclay);

TEACHING

3-6H / year in Master

EDITORIAL ACTIVITY

I review manuscripts for Plant Cell, Plant physiology, Plos Genetics, Nature plants, Trends in Plant Sciences and New Phytologist.

SCIENTIFIC PRODUCTION

EXTERNAL LINKS

Twitter: @andreas_niebel

HOBBY

Cycling, running, swimming, triathlon, mountain hiking

Andreas Niebel
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Phone +33 (0) 61 28 50 51

Key words

Symbiosis Legume- Rhizobium , Medicago truncatula , Sinorhizobium meliloti , electron microscopy

Marie-Christine Auriac

IE CNRS

ENOD team

RESEARCH SUBJECTS

Biotic interactions of plants with symbionts and pathogens, studied by cytology and microscopy approaches

TRAINING

PhD in plant biology and physiology - Training in light and electron microscopy

COLLABORATIONS

- Mireille Chabaud (LIPME, SPI team), on the Sunflower / Orobanche interaction

- Jean-Marc Routaboul (LIPME, SIX team), on the visualization of Brassicaceae hydathodes.

SCIENTIFIC PRODUCTION

Anne Bennion
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Phone +33(0) 61 28 55 08

Key words

Legume-Rhizobium symbiosisMedicago truncatulaSinorhizobium meliloti, molecular microbiology, synthetic biology

Anne Bennion

Visiting PhD student

ENOD team

RESEARCH SUBJECTS

Rhizobium-legume symbiosis, molecular microbiology, synthetic biology

TRAINING

Bachelor in Molecular Biology at Brigham Young University (2019),

PhD candidate at the International Max Planck Research School for Environmental, Cellular and Molecular Microbiology

COLLABORATIONS

Ludwig Maximilian University Munich (DE),

SynMikro - Center for Synthetic Microbiology, Marburg (DE)

LIPME, Toulouse (FR)

SCIENTIFIC PRODUCTION

Wang, L. Xu, G., Li, L., Ruan, M., Bennion, A., Wang, GL, Li, R., Qu, S. (2023). The OsBDR1-MPK3 module negatively regulates blast resistance by suppressing the jasmonate signaling and terpenoid biosynthesis pathway. Proceedings of the National Academy of Sciences 120:12. DOI:10.1073/pnas.2211102120

 

Li, R., Schuman, MC, Wang, Y., Llorca, LC, Bing, J., Bennion, A., Halitschke, R., and Baldwin, IT (2018). Jasmonate signaling makes flowers attractive to pollinators and repellant to florivores in nature. Journal of Integrative Plant Biology 60:190-194. DOI:10.1111/jipb.12607

Marie-Christine Auriac
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Matthias Benoit

CRCN INRAE

ENOD team

RESEARCH SUBJECTS

Epigenetic regulation of plant-microbe symbioses: We study the role of epigenetic modifications and regulators in the establishment of symbioses between plants and microorganisms. In particular, we explore the molecular mechanisms by which long non-coding RNAs participate in the remodeling of transcription and chromatin structure during the development of the nodule, a symbiotic nitrogen-fixing organ produced by the symbiosis between legumes and rhizobia bacteria. For this, we combine approaches of molecular biology, genetics, transcriptomics, epigenomics, and microscopy.

Phone +33 (0) 61 28 50 51

TRAINING

Key words

Epigenetics, genomics, chromatin, gene expression, non-coding RNAs, transposable elements, symbioses, Medicago , rhizobia

Matthias Benoit obtained his PhD in Physiology and Molecular Genetics in the Genetics, Reproduction and Development laboratory (GReD, Clermont-Ferrand, France), supervised by Dr. Aline V. Probst. His work has demonstrated the importance of histone variants in the organization of chromatin during developmental transitions in plants, in particular during the early development of the model plant Arabidopsis thaliana. He then joined The Sainsbury Laboratory Cambridge University (SLCU, Cambridge, UK) and the team of Jurek Paszkowski to study the epigenetic and environmental mechanisms involved in the control of transposable elements in cultivated plants. Matthias completed his training by joining the team of Zach Lippman (Cold Spring Harbor Laboratory, New York, USA) to conduct work on the importance of epigenetic and structural variation during the domestication and evolutionary history of the tomato.

COLLABORATIONS

- Marco Catoni (University of Birmingham, UK);

- Hajk-Georg Drost (Max Planck Institute for Biology Tubingen, Germany);

- Frédéric Pontvianne (LGDP Perpignan, France);

- Sébastien Carrère (LIPME, Toulouse, France).

EDITORIAL ACTIVITY

Assistant Features Editor for The Plant Cell (2020-2021).

Plantae Fellow (2017-2019)

 

Reviewer for PLOS Genetics, The Plant Cell, Journal of Experimental Botany, New Phytologist, BMC Biology, BMC Genomics, among others.

 

https://publons.com/researcher/3758361/matthias-benoit/

SCIENTIFIC PRODUCTION

EXTERNAL LINKS

Twitter: @Matt_Ben_

ResearchGate: www.researchgate.net/profile/Matthias-Benoit

Matthias Benoit
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Phone  +33 (0) 61 28 53 27

Key words

NF-YA1, pioneer transcription factor , chromatin modifier, Proximity labeling

Thi Thu Dang

Post-doctoral fellow

ENOD team

RESEARCH SUBJECTS

I am working on the pioneer transcription factor NF-YA1 in symbiotic nodules

TRAINING

Thi Thu Dang did her PhD in the lab of Plant Molecular Genetics, Nara Institute of Science and Technology (NAIST, Japan), 2009-2013. Her PhD project was to characterize the Constitutively Active OsRac1 (CA-OsRac1), a master regulator in rice immunity, generated by homologous recombination. From 2015-2017, she then did her first postdoc in the lab of Plant Immunity Signaling, Shanghai Center for Plant Stress Biology, China. There, she continued to study the down-stream regulator of OsRac1, called OsGAPC3, which plays an important role in NO-pathway and histone modification. From 2017 to 2020, she joined the Conserto team, INRAE, Angers for her second postdoc. The project focused on the identification of the protein network regulating ABI4, a transcription factor controlling seed chlorophyll content and longevity. Since October 2020, Thu joined the ENOD group to work on the “PIOSYM” ANR project aiming to evaluate the importance of NF-YA1 as a pioneer transcription factor regulating the formation of symbiotic nodules in Medicago.

RECENT FUNDING

ANR PIOSYM (2020-2023)

SCIENTIFIC PRODUCTION

  • Thu Thi Dang, Zenpei Shimatani, Yoji Kawano, Rie Terada, Ko Shimamoto. Gene editing a constitutively active OsRac1 by homologous recombination-based gene targeting induces immune responses in rice. Plant and Cell Physiology, Volume 54, Issue 12, December 2013, Pages 2058–2070

  • Julia Zinsmeister, Souha Berriri, Denise Puntel Basso, Benoit Ly-Vu, Thi-Thu Dang, David Lalanne, Edvaldo Aparecido Amaral da Silva, Olivier Leprince, Julia Buitink. The seed-specific heat shock factor A9 regulates the depth of dormancy in Medicago truncatula seeds via ABA signaling. Plant Cell Environ. 2020;1–15.

  • Akira Akamatsu, Masayuki Fujiwara, Satoshi Hamada, Megumi Wakabayashi, Ai Yao, Qiong Wang, Ken-ichi Kosami, Thu Thi Dang, Takako Kaneko-Kawano, Ko Shimamoto, Yoji Kawano. The Small GTPase OsRac1 forms two distinct immune receptor complexes containing the PRR OsCERK1 and the NLR Pit. July 2020. Plant and Cell Physiology. DOI: 10.1101/2020.07.01.183301

EXTERNAL LINKS

HOBBY

Photography, Calligraphy

Thi Thu Dang
Anaïs Delers
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Phone  +33 (0) 61 28 50 51

Anaïs Delers

PhD student

ENOD team

RESEARCH SUBJECTS

Study of the role of plant cell polarity proteins in legume-rhizobia symbioses

TRAINING

Master of Plant Biology ADAM (Adaptations, development, plant breeding, in association with microorganisms) at the Paul Sabatier University of Toulouse (2020-2022)

Key words

Legumes, symbiosis, rhizobia, cell polarity

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Phone  +33 (0) 61 28 53 24

Key words

Symbiosis Rhizobium - Legumes, infection threads, in vivo confocal microscopy, Medicago truncatula , Sinorhizobium meliloti , endosymbioses, cell biology

Joëlle Fournier

CRHC CNRS

ENOD team

RESEARCH SUBJECTS

Cellular mechanisms of rhizobial colonization of legumes

TRAINING

PhD from the University of Toulouse in Plant Physiology (1989)

RECENT FUNDING

Live-Switch PRCI-ANR (2020-2023); CREPE-SPE-INRAE (2020-2022)

COLLABORATIONS

A. Becker (SYNMIKRO, Marburg, Germany); M. Marín (LMU, Munich, Germany); F. Cartieaux, V. Hocher & S. Svistoonoff (LSTM, Montpellier); R. Peyraud (iMEAN, Toulouse).

EDUCATION

Contribution to the ADAM Master 2 (Adaptation, Development and Improvement of Plants in the Presence of Microorganisms) of the University of Toulouse III

SCIENTIFIC PRODUCTION

Joëlle Fournier
Lisa Frances
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Phone  +33 (0) 61 28 53 24

Key words

Symbiosis Legume- Rhizobium , molecular biology, cloning, plant cultivation, plant transformation

Lisa Frances

TREX INRAE

ENOD team

RESEARCH SUBJECTS

Legume- Rhizobium symbiosis . I participate in the research activities of the team in the theme "regulation and dynamics of rhizobial infection"

TRAINING

DUT in Biological Engineering (Agronomy) in 1999, then University Diploma in Plant Biotechnology (2000), University of Perpignan

SCIENTIFIC PRODUCTION

Ambre Guillory
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Phone  +33 (0) 561 28 53 24

Ambre Guillory

Post-doctoral fellow

ENOD team

RESEARCH SUBJECTS

Nitrogen-fixing symbiosis, cell biology, in vivo confocal microscopy

TRAINING

Key words

Endosymbiosis, Cell Biology, Medicago truncatula / Sinorhizobium meliloti interaction model, Confocal microscopy in vivo, Infection process

Bachelor in cell biology and physiology (Paris VII, 2015),

Master in plant sciences (Paris VII, 2017),

PhD in plant biology (Paris Saclay, 2020)

COLLABORATIONS

Macarena Marín, LMU, Institute of Genetics, Munich

Anke Becker, UMR, SYNMIKRO and Faculty of Biology, Marburg

SCIENTIFIC PRODUCTION

https://orcid.org/0000-0002-5836-4582

- Guillory A, Bonhomme S. Methods for Medium-Scale Study of Biological Effects of Strigolactone-Like Molecules on the Moss Physcomitrium (Physcomitrella) patens. Methods Mol Biol. 2021; 2309: 143-155. doi: 10.1007 / 978-1-0716-1429-7_12. PMID: 34028685. 

- Lopez-Obando M, Guillory A et al. The Physcomitrium (Physcomitrella) patens PpKAI2L receptors for strigolactones and related compounds highlight MAX2 dependent and independent pathways, bioRxiv 2020.11.24.395954; doi: https://doi.org/10.1101/2020.11.24.395954

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Phone +33 (0) 61 28 53 27

Key words

Development of symbiotic nodules, transcription factors, non-coding RNAs, transcriptional and epigenetic regulation of odulation, microscopy, cytology techniques, Technovit

Agnes Lepage

AI CNRS

ENOD

RESEARCH SUBJECTS

I participate in the work on the genetic and epigenetic control of the development of symbiotic nodules in Medicago truncatula , within the framework of projects led by Andreas Niebel (PIOSYM, NOCOSYM, MYCONODISLAND)

TRAINING

Training

SCIENTIFIC PRODUCTION

Agnès Lepage

Firstname name

Post-doctoral fellow

ENOD

RESEARCH SUBJECTS

TRAINING

Phone +33(0)61 28 55 08

Key words

RECENT FUNDING

SCIENTIFIC PRODUCTION

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