Within the REACH team, we are interested in the genetic basis of interactions between host plants and phytopathogenic microorganisms. In particular, we study the molecular mechanisms that enable immune intracellular NLRs (Nod-Like Receptors) to specifically detect phytopathogenic bacterial effectors and activate plant immune responses. The perception and signaling functions of NLRs are frequently negatively impacted by temperature increases, through mechanisms that remain largely unknown. To increase the resilience of the plant immune system to climate change, we are also exploiting the natural genetic diversity of model host plants (Arabidopsis thaliana, tomato) to identify and characterize thermotolerant immune components that confer robust resistance responses in an environment subject to fluctuating pathogens. The knowledge gained from this work will contribute to the future development of new control strategies for more environmentally friendly sustainable agriculture.
The team addresses a range of topics, mainly centered around four major scientific questions:
- What are the virulence functions of type III bacterial effectors of the YopJ acetyltransferase family?
- What are the molecular bases of NLR thermotolerance?
- What are the functions of TIR signaling domain proteins in the activation of plant immune responses at high temperatures?
- Can we identify other mechanisms that can confer thermoresilient immunity ?
MEMBERS
Beatriz Viana
PhD student
PUBLICATIONS
HAQ F., CAMUEL A., CARCAGNO M., BIONDI EG., PACQUIT V., DESLANDES L., GIRAUD E., MERGAERT P. (2025). The rhizobial type III effectors ErnA and Sup3 hijack the SUMOylation pathway to trigger nodule formation in Aeschynomene species. New Phytol. Aug;247(4):1826-1836. doi: 10.1111/nph.70334. Epub 2025 Jun 22. hal-05150211v1.
MONGE-WALERYSZAK L., GIRARD M., CARCAGNO M., CULERRIER R., VICEDO C., MARTINEZ Y., VERIN C., COUTE Y., PACQUIT V. C, DESLANDES L. C (2025). Three ARID proteins involved in chromatin remodeling PEAT complexes are targeted by the Ralstonia solanacearum effector PopP2 and contribute to bacterial wilt disease. Plant J. 2025 May;122(3):e70205. doi: 10.1111/tpj.70205. hal-05090319v1.
DEMONT H, REMBLIERE C, CULERRIER RAPHAËL, SAUVAGET M., DESLANDES L, BERNOUX M. C (2025) Downstream signaling induced by several plant Toll/interleukin-1 receptor-containing immune proteins is stable at elevated temperature. Cell Reports. 44(3) doi.org/10.1016/j.celrep.2025.115326. hal-04979086v1.
LAUBER E., GONZALEZ-FUENTE M., ESCOUBOUE M., VICEDO C., LUNEAU J., POUZET C., JAUNEAU A., GRIS C., ZHANG Z.-M., PICHEREAUX C., CARRERE S., DESLANDES L.C, NOEL L.C (2024). Bacterial host adaptation through sequence and structural variations of a single type III effector gene. iScience, 27 (3), 109224, 10.1016/j.isci.2024.109224, https://hal.archives-ouvertes.fr/hal-04664466
CHEN J., ZHANG X., BERNOUX M., RATHJEN J., DODDS P. (2024). Plant Toll/interleukin-1 receptor/resistance protein domains physically associate with enhanced disease susceptibility1 family proteins in immune signaling. iScience, 27 (2), 108817, 10.1016/j.isci.2024.108817, https://hal.archives-ouvertes.fr/hal-04648296
DESAINT H., GIGLI A., BELNY A., CASSAN-WANG H., MARTINEZ Y., VAILLEAU F., MOUNET F., VERNHETTES S., BERTHOME R., MARCHETTI M.C. (2024). Reshaping the Primary Cell Wall: Dual Effects on Plant Resistance to Ralstonia solanacearum and Heat Stress Response. Molecular Plant-Microbe Interactions, 37 (8), 619-634, 10.1094/MPMI-05-24-0059-R, https://hal.archives-ouvertes.fr/hal-04719242
KIM H., KIM J., CHOI D. S., KIM M., DESLANDES L., JAYARAMAN J., SOHN K. H. (2024). Molecular basis for the interference of the Arabidopsis WRKY54 ‐mediated immune response by two sequence‐unrelated bacterial effectors. The Plant Journal, 118 (3), 839-855, 10.1111/tpj.16639, https://hal.archives-ouvertes.fr/hal-04609779
DEMIRJIAN C., VAILLEAU F., BERTHOME R., ROUX F. (2023). Genome-wide association studies in plant pathosystems: success or failure?. Trends in Plant Science, 28 (4), 471-485, 10.1016/j.tplants.2022.11.006, https://hal.archives-ouvertes.fr/hal-04158280
BODELOT A., CHAVONET E., BRISSET M. N., DOUSSET N., RAVON E., HEINTZ C., BERTHOME R., ZAFFUTO M., KEMPF M., FOULON M., MARION E., VERGNE E., DEGRAVE A. (2023). Overexpression of an apple broad range agglutinating lectin does not promote in planta resistance to fire blight and bacterial wilt. Journal of Plant Pathology, , 10.1007/s42161-023-01479-x, https://hal.archives-ouvertes.fr/hal-04289707
CAMUEL A., TEULET A., CARCAGNO M., HAQ F., PACQUIT V., GULLY D., PERVENT M., CHAINTREUIL C., FARDOUX J., HORTA-ARAUJO N., OKAZAKI S., RATU S., GUEYE F., ZILLI J., NOUWEN N., ARRIGHI J.-F., LUO H., MERGAERT P., DESLANDES L., GIRAUD E. (2023). Widespread Bradyrhizobium distribution of diverse Type III effectors that trigger legume nodulation in the absence of Nod factor. The International Society of Microbiologial Ecology Journal, 17, 1416-1429, 10.1038/s41396-023-01458-1, https://hal.archives-ouvertes.fr/hal-04189542
LANDRY D., MILA I., SABBAGH C. R. R., ZAFFUTO M., POUZET C., TREMOUSAYGUE D., DABOS P., DESLANDES L.C, PEETERS N.C (2023). An NLR integrated domain toolkit to identify plant pathogen effector targets. The Plant Journal, , 10.1111/tpj.16331, https://hal.archives-ouvertes.fr/hal-04187640
BERNOUX M.C, CHEN J., ZHANG X., NEWELL K., HU J., DESLANDES L., DODDS P. (2023). Subcellular localization requirements and specificities for plant immune receptor Toll‐interleukin‐1 receptor (TIR) signaling. The Plant Journal, 114 (6), 1319-1337, 10.1111/tpj.16195, https://hal.archives-ouvertes.fr/hal-04035820
DEMIRJIAN C., RAZAVI N., YU G., MAYJONADE B., ZHANG L., LONJON F., CHARDON F., CARRERE S., GOUZY J., GENIN S., MACHO A., ROUX F., BERTHOME R., VAILLEAU F. (2023). An atypical NLR gene confers bacterial wilt susceptibility in Arabidopsis. Plant Communications, 4 (5), 100607, 10.1016/j.xplc.2023.100607, https://hal.archives-ouvertes.fr/hal-04234790
BECKER C., BERTHOME R., DELAVAULT P., FLUTRE T., FREVILLE H., GIBOT-LECLERC S., LE CORRE V., MOREL J. B., MOUTIER N., MUNOS S., RICHARD-MOLARD C., WESTWOOD J., COURTY P.-E., DE SAINT GERMAIN A., LOUARN G., ROUX F. (2023). The ecologically relevant genetics of plant-plant interactions. Trends in Plant Science, 28 (1), 31-42, 10.1016/j.tplants.2022.08.014, https://hal.archives-ouvertes.fr/hal-03800896
BARTOLI C., BOIVIN S., MARCHETTI M., GRIS C., GASCIOLLI V., GASTON M., AURIAC M.C., DEBELLE F., COTTRET L., CARLIER A., MASSON C., LEPETIT M., LEFEBVRE B. (2022). Rhizobium leguminosarum symbiovar viciae strains are natural wheat endophytes that can stimulate root development. Environ Microbiol. 24(11):5509-5523. doi: 10.1111/1462-2920.16148. http://hal.inrae.fr/hal-02967159v1
DEMIRJIAN C.*, RAZAVI N.*, DESAINT H., LONJON F., GENIN S., ROUX F., BERTHOME R.*, VAILLEAU F.* (2022). Study of natural diversity in response to a key pathogenicity regulator of Ralstonia solanacearum reveals new susceptibility genes in Arabidopsis thaliana. Molecular Plant Pathology, 23 (3), 321-338, 10.1111/mpp.13135, https://hal.archives-ouvertes.fr/hal-03621760
LUNEAU J., CERUTTI A., ROUX B., CARRERE S., JARDINAUD M., GAILLAC A., GRIS C., LAUBER E., BERTHOME R., ARLAT M., BOULANGER A., NOEL L., ARLAT M. (2022). Xanthomonas transcriptome inside cauliflower hydathodes reveals bacterial virulence strategies and physiological adaptations at early infection stages. Molecular Plant Pathology, 23 (2), 159-174, 10.1111/mpp.13117, https://hal.archives-ouvertes.fr/hal-03580527
DESAINT H., AOUN N., DESLANDES L., VAILLEAU F., ROUX F., BERTHOME R.C (2021). Fight hard or die trying: when plants face pathogens under heat stress. New Phytologist, 229 (2), 712-734, 10.1111/nph.16965, https://hal.archives-ouvertes.fr/hal-03176541
ZHU X., MAZARD J., ROBE E., PIGNOLY S., AGUILAR M., SAN CLEMENTE H., LAUBER E., BERTHOME R., GALAUD J.-P. (2021). The Same against Many: AtCML8, a Ca2+ Sensor Acting as a Positive Regulator of Defense Responses against Several Plant Pathogens. International Journal of Molecular Sciences, 22 (19), 10469, 10.3390/ijms221910469, https://hal.archives-ouvertes.fr/hal-03383369
XIA Y.*, ZOU R.*, ESCOUBOUE M.*, ZHONG L., ZHU C., POUZET C., WU X., WANG Y., LV G., ZHOU H., SUN P., DING K., DESLANDES L.C, YUAN S., ZHANG Z.-M. (2021). Secondary-structure switch regulates the substrate binding of a YopJ family acetyltransferase. Nature Communications, 12 (1), 10 p., 10.1038/s41467-021-26183-1, https://hal.archives-ouvertes.fr/hal-03511076
DELPLACE F., HUARD‐CHAUVEAU C., BERTHOME R., ROBY D. (2021). Network organization of the plant immune system: from pathogen perception to robust defense induction. The Plant Journal, , 24 p., 10.1111/tpj.15462, https://hal.archives-ouvertes.fr/hal-03533890
DONGUS J. A., BHANDARI D., PATEL M., ARCHER L., DIJKGRAAF L., DESLANDES L., SHAH J., PARKER J. (2020). Arabidopsis PAD4 lipase-like domain is sufficient for resistance to green peach aphid. Molecular Plant-Microbe Interactions, 33 (2), 328-335, 10.1094/MPMI-08-19-0245-R, https://hal.archives-ouvertes.fr/hal-02409700
ZULUAGA P. A., BIDZINSKI P., CHANCLUD E., DUCASSE A., CAYROL B., GOMEZ SELVARAJ M., ISHITANI M., JAUNEAU A., DESLANDES L., KROJ T., MICHEL C., SZUREK B., KOEBNIK R., MOREL J.-B. (2020). The Rice DNA-Binding Protein ZBED Controls Stress Regulators and Maintains Disease Resistance After a Mild Drought. Frontiers in Plant Science, 11, 1265, 10.3389/fpls.2020.01265, https://hal.archives-ouvertes.fr/hal-02927839
AOUN N., DESAINT H., BOYRIE L., BONHOMME M., DESLANDES L., BERTHOME R.C, ROUX F.C (2020). A complex network of additive and epistatic quantitative trait loci underlies natural variation of Arabidopsis thaliana quantitative disease resistance to Ralstonia solanacearum under heat stress. Molecular Plant Pathology, 21 (11), 1405-1420, 10.1111/mpp.12964, https://hal.archives-ouvertes.fr/hal-02948127
BACETE L., MELIDA H., LOPEZ G., DABOS P., TREMOUSAYGUE D., DENANCE N., MIEDES E., BULONE V., GOFFNER D., MOLINA A. (2020). Arabidopsis Response Regulator 6 (ARR6) Modulates Plant Cell-Wall Composition and Disease Resistance. Molecular Plant-Microbe Interactions, 33 (5), 767-780, 10.1094/MPMI-12-19-0341-R, https://hal.archives-ouvertes.fr/hal-03023355
LANDRY D., GONZALEZ‐FUENTE M., DESLANDES L.C, PEETERS N.C (2020). The large, diverse, and robust arsenal of Ralstonia solanacearum type III effectors and their in planta functions. Molecular Plant Pathology, 21 (10), 1377-1388, 10.1111/mpp.12977, https://hal.archives-ouvertes.fr/hal-02923763
MALBERT B., BURGER M., LOPEZ OBANDO M., BAUDRY K., LAUNAY-AVON A., HARTEL B., VERBITSKIY D., JORG A., BERTHOME R., LURIN C., TAKENAKA M., DELANNOY E. (2020). The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins. Plants, 9 (2), 280, 10.3390/plants9020280, https://hal.archives-ouvertes.fr/hal-02628277
DUXBURY Z., WANG S., MACKENZIE C., TENTHOREY J., ZHANG X., HUH S., HU L., HILL L., NGOU P., DING P., CHEN J., MA Y., GUO H., CASTEL B., MOSCHOU P., BERNOUX M., DODDS P., VANCE R., JONES J. (2020). Induced proximity of a TIR signaling domain on a plant-mammalian NLR chimera activates defense in plants. Proceedings of the National Academy of Sciences of the United States of America, 117 (31), 18832-18839, 10.1073/pnas.2001185117, https://hal.archives-ouvertes.fr/hal-02975048
LEONHARDT N., DIVOL F., CHIARENZA S., DESCHAMPS S., RENAUD J., GIACALONE C., NUSSAUME L., BERTHOME R., PERET B. (2020). Tissue‐specific inactivation by cytosine deaminase/uracil phosphoribosyl transferase as a tool to study plant biology. The Plant Journal, 101 (3), 731-741, 10.1111/tpj.14569, https://hal.archives-ouvertes.fr/cea-02363540
FORMER MEMBERS
Dominique Tremousaygue
CRHC CNRS
Virginie Comorge
Doctorante
Adrien Belny
Doctorante
Céline Vicédo
IE CNRS
Héloïse Demont
Doctorante
Caitlin Griffiths
CDD IE
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Mélanie CARCAGNO
IE CDD
Lea Monge-Waleryszak
Doctorante