Laurent Deslandes
DR2 CNRS
team leader
RESEARCH SUBJECTS
Since 2016, I have been leading the REACH team (Dynamics of plant immune responses in a context of climate change), which currently comprises five permanent members (one CNRS DR2, one INRAE DR2, one CNRS CRCN, one INRAE CRHC, 1 MCF-UT, 1 IEHC-CNRS) and various contract staff (PhD students, fixed-term contracts, etc.).
My research focuses on elucidating the different stages of the molecular dialogue that takes place between phytopathogenic bacteria and their host plants. Among the key players in the plant immune system are intracellular Nod-Like Receptors (NLRs), which are capable of specifically recognizing effectors delivered by pathogens within plant cells. In addition to studying the perception and signaling functions of NLRs, I am also interested in the deciphering of the virulence functions of type III effectors produced by various phytopathogenic bacteria, particularly acetyltransferases belonging to the YopJ family.
LIPME - Laboratoire des interactions plantes-microbes-environnement
UMR CNRS-INRA 2594/441
24, chemin de borderouge- Auzeville
CS 52627
31326 Castanet-Tolosan Cedex
France
Phone : +33 561285509
plant immunity
NLR-type immune receptors
climate change
signaling, type III effectors
mechanisms of interference with the host epigenome.
TRAINING
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Since 2018: CNRS CRCN, LIPME, Toulouse, France
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2012-2018: Junior research scientist, CSIRO Agriculture, Canberra, Australia
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2012-2015: ARC DECRA fellow, CSIRO Agriculture, Canberra, Australia
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2008-2012: Postdoc fellow, CSIRO Agriculture, Canberra, Australia
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2008: PhD degree in Plant sciences, University Paul Sabatier, LIPM, Toulouse, France
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2004: Master Degree (DEA) « Plant-Microbe Interaction »/Engineer degree Agro Paris-Tech, University Paris VI, Paris XI, AgroParis Tech, France
COLLABORATIONS
Zhi-Min Zang (Jinan University, China), Moussa Benhamed/Cécile Raynaud (IPS2, Paris-Saclay), Lionel Navarro (IBENS, ENS), Eric Giraud (PHIM, Montpellier)…
RECENT FUNDING
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SCIENTIFIC PRODUCTION
ORCID : https://orcid.org/0000-0003-1974-5144
Haq F, Camuel A, Carcagno M, Biondi EG, Pacquit V, Deslandes, L, Giraud E, Mergaert P. The rhizobial type III effectors ErnA and Sup3 hijack the SUMOylation pathway to trigger nodule formation in Aeschynomene species. New Phytol. 2025 Aug;247(4):1826-1836. doi: 10.1111/nph.70334. Epub 2025 Jun 22. hal-05150211v1.
Monge-Waleryszak L, Girard M, Carcagno M, Culerrier R, Vicédo C, Martinez Y, Vérin C, Couté Y, Pacquit V, *Deslandes, L. Three ARID proteins involved in chromatin remodeling PEAT complexes are targeted by the Ralstonia solanacearum effector PopP2 and contribute to bacterial wilt disease. Plant J. 2025 May;122(3):e70205. doi: 10.1111/tpj.70205. hal-05090319v1.
Demont H, Remblière C, Culerrier R, Sauvaget M., Deslandes, L, Bernoux M. Downstream signaling induced by several plant Toll/interleukin-1 receptor-containing immune proteins is stable at elevated temperature. Cell Reports. 2025 44(3) doi.org/10.1016/j.celrep.2025.115326. hal-04979086v1.
Lauber E, González-Fuente M, Escouboué M, Vicédo C, Luneau JS, Pouzet C, Jauneau A, Gris C, Zhang ZM, Pichereaux C, Carrère S, *Deslandes, L, *Noël LD. Bacterial host adaptation through sequence and structural variations of a single type III effector gene. iScience. 2024 27(3):109224. hal-04664466v1.
Kim H, Kim J, Choi DS, Kim MS, Deslandes, L, Jayaraman J, Sohn KH. Molecular basis for the interference of the Arabidopsis WRKY54-mediated immune response by two sequence-unrelated bacterial effectors. Plant J. 2024 118(3):839-855. hal-04609779v1.
Bernoux M, Chen J, Zhang X, Newell K, Hu J, Deslandes, L, Dodds P. Subcellular localization requirements and specificities for plant immune receptor Toll-interleukin-1 receptor signaling. Plant J. 2023 114(6):1319-1337. hal-04035820v1.
Camuel A, Teulet A, Carcagno M, Haq F, Pacquit V, Gully D, Pervent M, Chaintreuil C, Fardoux J, Horta-Araujo N, Okazaki S, Ratu STN, Gueye F, Zilli J, Nouwen N, Arrighi JF, Luo H, Mergaert P, Deslandes, L, Giraud E. Widespread Bradyrhizobium distribution of diverse Type III effectors that trigger legume nodulation in the absence of Nod factor. ISME J. 2023 17(9):1416-1429. hal-04189542v1.
Xia Y, Zou R, Escouboué M, Zhong L, Zhu C, Pouzet C, Wu X, Wang Y, Lv G, Zhou H, Sun P, *Ding K, *Deslandes, L, *Yuan S, *Zhang ZM. Secondary-structure switch regulates the substrate binding of a YopJ family acetyltransferase. Nat Commun. 2021 12:5969. doi: 10.1038/s41467-021-26183-1. hal-03749090.
Desaint H, Aoun N, Deslandes, L, Vailleau F, Roux F, Berthomé R. Fight hard or die trying: when plants face pathogens under heat stress. New Phytol. 2021 229:712-734. doi: 10.1111/nph.16965. hal-03176541.
Landry D., Mila I., Sabbagh, Rubenstein R., Zaffuto M., Pouzet C., Tremousaygue D., Dabos P., *Deslandes, L., *Peeters N. An NLR Integrated Decoy toolkit to identify plant pathogen effector targets. Plant J. 115. 2021. 1443-1457. hal-03812535v1.
Landry D, González-Fuente M, Deslandes, L, Peeters N. The large, diverse, and robust arsenal of Ralstonia solanacearum type III effectors and their in planta functions. Mol Plant Pathol. 2020 10:1377-1388. doi: 10.1111/mpp.12977. hal-02923763.
Zuluaga AP, Bidzinski P, Chanclud E, Ducasse A, Cayrol B, Gomez Selvaraj M, Ishitani M, Jauneau A, Deslandes, L, Kroj T, Michel C, Szurek B, Koebnik R, Morel JB. The Rice DNA-Binding Protein ZBED Controls Stress Regulators and Maintains Disease Resistance After a Mild Drought. Front Plant Sci. 2020 11:1265. doi: 10.3389/fpls.2020.01265. hal-02927839.
Aoun N, Desaint H, Boyrie L, Bonhomme M, Deslandes, L, Berthomé R, Roux F. A complex network of additive and epistatic quantitative trait loci underlies natural variation of Arabidopsis thaliana quantitative disease resistance to Ralstonia solanacearum under heat stress. Mol Plant Pathol. 2020 11:1405-1420. doi: 10.1111/mpp.12964. hal-02948127.
Grund E., Tremousaygue D., and *Deslandes, L. (2019). Plant NLRs with Integrated Domains: Unity Makes Strength. Plant Physiol 179, 1227-1235. hal-02387374v1
Dongus JA, Bhandari DD, Patel M, Archer L, Dijkgraaf L, Deslandes, L, Shah J, Parker JE. (2019). The Arabidopsis PAD4 Lipase-Like Domain Is Sufficient for Resistance to Green Peach Aphid. Mol Plant Microbe Interact. 27:MPMI08190245R. doi: 10.1094/MPMI-08-19-0245-R. hal-02409700.
Teulet A, Busset N, Fardoux J, Gully D, Chaintreuil C, Cartieaux F, Jauneau A, Comorge V, Okazaki S, Kaneko T, Gressent F, Nouwen N, Arrighi JF, Koebnik R, Mergaert P, Deslandes, L, Giraud E. (2019). The rhizobial type III effector ErnA confers the ability to form nodules in legumes. Proc Natl Acad Sci USA 22;116(43):21758-21768. doi/10.1073/pnas.1904456116. hal-02318403v1.
Aoun N, Tauleigne L, Lonjon F, Deslandes, L, Vailleau F, Roux F and Berthomé R, (2017). Quantitative Disease Resistance in the context of global warming: genetic basis of new resistance mechanisms to Ralstonia solanacearum. Front. Plant Sci. 8:1387. doi: 10.3389/fpls.2017.01387.
Henry, E., Toruño, T.Y., Jauneau, A., Deslandes, L., and Coaker, G. (2017). Direct and Indirect Visualization of Bacterial Effector Delivery into Diverse Plant Cell Types during Infection. Plant Cell 29, 1555-1570. hal-01606044v1.
Camborde, L., Jauneau, A., Brière, C., Deslandes, L., Dumas, B., and Gaulin, E. (2017). Detection of nucleic acid-protein interactions in plant leaves using fluorescence lifetime imaging microscopy. Nature Protocols 12, 1933-1950. hal-01607375v1.
Hanemian, M., Barlet, X., Sorin, C., Yadeta, K.A., Keller, H., Favery, B., Simon, R., Thomma, B.P., Hartmann, C., Crespi, M., Marco, Y., Tremousaygue, D., and *Deslandes, L. (2016). Arabidopsis CLAVATA1 and CLAVATA2 receptors contribute to Ralstonia solanacearum pathogenicity through a miR169-dependent pathway. New Phytol. 211, 502-515. hal-02636021v1.
LIPME - Laboratoire des interactions plantes-microbes-environnement
UMR CNRS-INRA 2594/441
24, chemin de borderouge- Auzeville
CS 52627
31326 Castanet-Tolosan Cedex
France
lant immunity
immune receptors
NLR, TIR, signaling abiotic stresses, temperature
Bernoux Maud
CRCN CNRS
deputy team leader
RESEARCH SUBJECTS
My scientific interest lies in understanding how plants interact with their environment, and more specifically, how they defend themselves against pathogenic attacks in the context of fluctuating climates. My research focuses on the functional study of canonical and non-canonical intracellular immune receptors of the NLR type. These receptors sometimes contain a TIR signaling domain, which is found across all kingdoms of life.
The main research questions are:
1. What is the impact of temperature and other abiotic stresses on NLR function and plant immunity?
2. What is the functional diversity of TIR domains in plants?
TRAINING
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Since 2018: CNRS CRCN, LIPME, Toulouse, France
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2012-2018: Junior research scientist, CSIRO Agriculture, Canberra, Australia
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2012-2015: ARC DECRA fellow, CSIRO Agriculture, Canberra, Australia
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2008-2012: Postdoc fellow, CSIRO Agriculture, Canberra, Australia
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2008: PhD degree in Plant sciences, University Paul Sabatier, LIPM, Toulouse, France
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2004: Master Degree (DEA) « Plant-Microbe Interaction »/Engineer degree Agro Paris-Tech, University Paris VI, Paris XI, AgroParis Tech, France
RECENT FUNDING
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2025-2029 : ANR PRME : NLResET (NLR-mediated Resilient Immune responses under Elevated Temperature)
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2021-2024 : INRAE département SPE : PIMs (Proximity labelling of plant IMmune receptor Signaling complexes under heat stress)
TEACHING
I teach plant-microbe interactions and plant immunity to master students :
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Since 2019 : master Agrobiosciences Plant microbe interactions (INP-ENSAT, Toulouse).
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Since 2020 : master Agrosciences, Université de Bordeaux
I occasionally give lectures in other master's programs to present my research (master Plant Sciences, Université Paris 7, master Biologie des plantes et micoorganismes associés, Université de Toulouse)
COMMITTEE MEMBER
Since 2020 : Labex TULIP scientific council
2021-2024 : LIPME lab council
Since 2021 : Scientific council INRAE MoDIP network (Molecular dialogue between plants and microbes)
EDITORIAL ACTIVITY
Since 2023 : Associate editor MPMI
SCIENTIFIC PRODUCTION
COMPLEMENTARY ACTIVITY
SInce 2019 : LIPME Gender equity committee
SCIENCE AND SOCIETY
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2019 : Pint of science Toulouse: « Immunitaire mon cher Watson »
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2021 : Hands on activities in a primary school (LIPME 40th anniversary)
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2022 : Publication of a science outreach article « Le système immunitaire des plantes » le Petit Illustré du CNRS/La dépêche du Midi.
LIPME - Laboratoire des interactions plantes-microbes-environnement
UMR CNRS-INRA 2594/441
24, chemin de borderouge- Auzeville
CS 52627
31326 Castanet-Tolosan Cedex
France
Richard Berthomé
DR INRAE
RESEARCH SUBJECTS
Robust resistances and alternatives to face pathogens under global warming context
Plant diseases are responsible for significant economic losses and are one of the major obstacles to global food security. The development of these diseases rely on three components: the host plant, the pathogen and the environment. The two later correspond respectively to the biotic and abiotic constraints the plant must face in its habitat. In addition to cultural practices and the integrated use of chemical and biological products to limit pathogen populations, the identification of new genetic sources of resistance is often the most effective eco-friendly way to fight against these diseases.
In the context of climate change, the various scenarios predict an increase in the frequency and intensity of extreme weather events. These upheavals are already modifying the geographical distribution of species and the ecosystems functioning. They would also contribute to the reduction of natural biodiversity, favour the emergence of new bio-aggressors and increase the frequency and severity of epidemics. Temperature is one of the climate parameters predicted to fluctuate the most by the end of the century. Worryingly, a growing number of studies show that elevated temperatures negatively affect the majority of known and used sources of pathogen resistance.
We are studying plant responses to combined biotic and abiotic constraints. More specifically, we focus our work on the impact of the temperature elevation or climate change on the plant-pathogen interaction. For this purpose, we use the resources and tools available for the Arabidopsis/tomato-R.solanacearum pathosystems. The projects are divided into three axes that aim at:
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Understanding the mechanisms involved in defense responses inhibition at elevated temperature while considering the two partners of the plant-pathogen interaction (with Marta Marchetti).
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Identifying and studying the genetic bases of robust resistance mechanisms remaing effecient at elevated temperature or under climate change conditions in field. We are exploring the natural diversity of Arabidopsis and tomato responses to the bacterium, using several approaches including association genetics and functional validations. We are also evaluating the impact of the genetic diversity of the pathogen (with L. Deslandes; M. Bernoux).
3) Assessing the importance of other environmental factors (root microbiota, plant-plant associations) in order to find alternatives to maintain the resistances effectiveness under climate change conditions
TRAINING
Recruited in 2001 at the Station de Génétique et d'Amélioration des Plantes (Institut Jean-Pierre Bourgin-INRA, Versailles) in the "Organelles et Reproduction" team, I first developed a project to study the involvement of mitochondria in the development and sexual reproduction of higher plants. In 2007, I joined the A. thaliana functional genomics team of the Plant Genomics Research Unit (URGV-INRA) in EVRY. My work focused on i) the study of the regulation of genome expression in a context of adaptation of the plant to its environment and more particularly in response to abiotic stresses (cell differentiation process, nitrogen deficiency, salt stress, organic pollutant) and ii) the study of the pyrimidine base recycling pathway and its manipulation in order to develop a system allowing access to the transcriptome of specific tissues. I joined the "Dynamics of plant resistance mechanisms and adaptation to global warming" team at LIPME, led by Laurent Deslandes in 20212. I am currently developing several projects that aim at 1) understanding the plant response to biotic and abiotic combined and at identifying and characterizing 2) robust genetic resistance mechanisms or 3) alternative solutions that would maintain the effectiveness of resistance mechanisms in the context fo global warming.
RECENT FUNDING
2023-2029 ANR PIA4 SOYSTAINABLE
2023-2025 Research Partnership INRAE-SYNGENTA ELEVATION
2022-2024 Research Partnership INRAE-SYNGENTA CRISP.
2021-2023 AAP SPE INRAE; INTeGRATION
2021-2023 Research Partnership INRAE-SYNGENTA: Project BURNED III
2021-2023 AAP SPE INRAE; INTeGRATION
2021 AAP FRAIB AWARE
2020-2022 Research Partnership INRAE-SYNGENTA: Project BURNED II.
2018-2021 ANR PRC CappTure
2018-2019 AO innovation Labex TULIP: project STARTER
2017-2021 Research Partnership INRAE-SYNGENTA: project BURNED
2017-2018 AO innovation Labex TULIP: projet RETHINk
2014-2015 AAP SPE INRA
2014 AAP FRAIB (Coll. D. Aldon LRSV)
SCIENTIFIC PRODUCTION
Publications related to current projects
Demirjian, C., Razavi, N., Desaint, H., Lonjon, F., Genin, S., Roux, F., Berthomé, R. & Vailleau, F. (2022). Study of natural diversity in response to a key pathogenicity regulator of Ralstonia solanacearum reveals new susceptibility genes in Arabidopsis thaliana. Molecular Plant Pathol., 23: 321-338. ⟨10.1111/mpp.13135⟩. ⟨hal-03621760⟩
Delplace, F., Huard-Chauveau, C., Berthomé, R. & Roby, D. (2022). Network organization of the plant immune system: from pathogen perception to robust defense induction. The Plant Journal, 109, 447– 470. ⟨10.1111/tpj.15462⟩. ⟨hal-03533890⟩.
Desaint, H., Aoun, N., Deslandes, L., Vailleau, F., Roux, F. & Berthomé, R. (2021). Fight hard or die trying: when plants face pathogens under heat stress. New Phytol. 229: 712-34. ⟨10.1111/nph.16965⟩.⟨hal-03176541⟩
Zhu, X., Mazard, J., Robe, E., Pignoly, S., Aguilar, M., San Clemente, H., Lauber, E., Berthomé, R. & Galaud, J.P. (2021). The same against many: AtCML8 acts as a common regulator of defense responses to several species of phytopathogens. Int. J. Mol. Sci., MDPI, 22: 10469. ⟨10.3390/ijms221910469⟩. ⟨hal-03383369⟩.
Aoun, N., Desaint, H., Boyrie, L., Bonhomme, M., Deslandes, L., Berthomé, R. & Roux, F. (2020). A complex network of additive and epistatic QTLs underlies natural variation of Arabidopsis thaliana quantitative disease resistance to Ralstonia solanacearum under heat stress. Molecular Plant Pathol., 21:1405-20. ⟨10.1111/mpp.12964⟩. ⟨hal-02948127⟩.
Berthomé, R., Moury, B., Lefebvre, V. & Fagard, M. (2020). Chapitre coordonné « Effets des changements environnementaux sur l'immunité végétale » dans « L'immunité végétale: comment les plantes résistent aux maladies » (ed Lannou, C., Roby, D., Ravigné, V., Hannachi, M., Moury, B.), Quæ, Versailles, 392 p. ⟨hal-03130970⟩.
Aoun, N., Tauleigne, L., Lonjon, F., Deslandes, L., Vailleau, F., Roux, F. & Berthomé, R. (2017). Quantitative Disease Resistance under Elevated Temperature: Genetic Basis of New Resistance Mechanisms to Ralstonia solanacearum. Front Plant Sci. 8:1387. ⟨10.3389/fpls.2017.01387⟩. ⟨hal-01608184⟩.
LIPME - Laboratoire des interactions plantes-microbes-environnement
UMR CNRS-INRA 2594/441
24, chemin de borderouge- Auzeville
CS 52627
31326 Castanet-Tolosan Cedex
France
Key words
Marta Marchetti
CRHC INRAE
RESEARCH SUBJECTS
Immune responses in Arabidopsis thaliana roots: specificities of plant response to R. solanacearum and effect of heat stress
My field of research is focused on the interaction of bacterial pathogens with plants at the cellular and molecular level with, in particular, a cellular biological approach. My main interest is to better understand the plant root immunity to bacterial pathogens and the effects of climate change on host-pathogen interaction.
TRAINING
My scientific career started with a thesis in the Department of Immunology and Molecular Biology at the Chiron Vaccine Spa in Italy (Siena) on the development of a mouse model for the infection of the human pathogen, Helicobacter pylori. I then did a first postdoctoral internship in the Department of Bacteriology and Mycology directed by P. Sansonetti at the Pasteur Institute on the understanding of the molecular mechanisms associated with the development of esophageal metaplasia or Barrett's syndrome.
During my second post-doctoral internship in the Department of Cell Dynamics and Compartmentalization at the Curie Institute in Paris, I worked on a project centered on the molecular mechanisms of the endocytic pathways and signaling of the Interferon receptor. In 2005, I was recruited at INRAE and joined the "Symbiotic functions, genomes, and evolution of rhizobia" team at LIPME. My interest was mainly guided by questions on the evolutionary history of rhizobia and the understanding of the molecular mechanisms, which allowed their emergence.
In 2019 I joined the "Dynamics of the immune response and adaptation to climate change" team led by Laurent Deslandes.
I am interested in studying the immune responses of plants to R. solanacearum at the natural pathway of entry of R. solanacearum, the root.
Roots represent an important opportunistic entryway for a number of soil pathogens. Amongst pathogenic organisms, vascular pathogens penetrate the root system to infect and cause symptoms within the aerial parts of host plants such as their leaves.
This is the case for the phytopathogenic vascular bacterium R. solanacearum. By working on the pathosystem composed of the phytopathogenic bacteria R. solanacearum and the model plant Arabidopsis thaliana, the goal of my research project is to investigate the response in A. thaliana roots challenged with R. solanacearum and the impact of elevated temperature to plant response.
RECENT FUNDING
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COLLABORATIONS
Collaborations
EDUCATION
Teachings
COMMITTEE MEMBER
Committee member
EDITORIAL ACTIVITY
Editorial activities
SCIENTIFIC PRODUCTION
Desaint H., Gigli A., Belny A., Cassan-Wang H., Martinez Y., Vailleau F., Mounet F., Vernhettes S.,
Berthomé R. and M. Marchetti. Reshaping the Primary Cell Wall: Dual Effects on Plant Resistance to Ralstonia solanacearum and Heat Stress Response doi.org/10.1094/MPMI-05-24-0059-R hal-04672689
Bartoli C., Boivin S., Marchetti M., Gris C., Gasciolli V., Gaston M., Auriac M-C., Cottret L., Carlier A., Masson-Boivin, Lepetit M., Lefebvre B. 2022. Rhizobium leguminosarum symbiovar viciae strains are natural wheat endophytes and can stimulate root development and colonization by arbuscular mycorrhizal fungi. Environ Microbiol 24:5509-5523. doi: 10.1111/1462-2920.16148 hal-02967159
Checcucci A. and . The rhizosphere talk show : the rhizobia on stage. Review. Frontiers in Agronomy 2:591494 doi: 10.3389/fagro.2020.591494. hal-04672681
Benoit Daubech, Véréna Poinsot, Agnieszka Klonowska, Delphine Capela, Clemence Chaintreuil, Lionel Moulin, Marta Marchetti, Catherine Masson-Boivin (2019-10-16). noeM, a New Nodulation Gene Involved in the Biosynthesis of Nod Factors with an Open-Chain Oxidized Terminal Residue and in the Symbiosis with Mimosa pudica. Molecular Plant-Microbe Interactions, 32 (12), MPMI-06-19-0168, https://dx.doi.org/10.1094/MPMI-06-19-0168-R, https://hal.inrae.fr/hal-02326461
Marta Marchetti, Camille Clerissi, Yasmine Yousfi, Carine Gris, Olivier Bouchez, Eduardo Rocha, Stéphane Cruveiller, Alain Jauneau, Delphine Capela, Catherine Masson (2017-04). Experimental evolution of rhizobia may lead to either extra- or intracellular symbiotic adaptation depending on the selection regime. Molecular Ecology, 26 (7), 1818-1831, https://dx.doi.org/10.1111/mec.13895, https://hal.inrae.fr/hal-01607703
Benoit Daubech, Philippe Remigi, Ginaini Grazielli Doin de Moura, Marta Marchetti, Cecile Pouzet, Marie-Christine Auriac, Chaitanya S. Gokhale, Catherine Masson-Boivin, Delphine Capela (2017). Spatio-temporal control of mutualism in legumes helps spread symbiotic nitrogen fixation. eLife, 6 e28683, https://dx.doi.org/10.7554/eLife.28683, https://hal.inrae.fr/hal-02618918
Delphine Capela, Marta Marchetti, Camille Clerissi, Anthony Perrier, Dorian Guetta, Carine Gris, Marc Valls, Alain Jauneau, Stéphane Cruveiller, Eduardo P. C. Rocha, Catherine Masson-Boivin (2017). Recruitment of a Lineage-Specific Virulence Regulatory Pathway Promotes Intracellular Infection by a Plant Pathogen Experimentally Evolved into a Legume Symbiont. Molecular Biology and Evolution, 34 (10), 2503-2521, https://dx.doi.org/10.1093/molbev/msx165, https://hal.inrae.fr/hal-02620519
Marta Marchetti, Alain Jauneau, Delphine Capela, Philippe Remigi, Carine Gris, Jacques Batut, Catherine Masson-Boivin (2014-09). Shaping Bacterial Symbiosis With Legumes by Experimental Evolution. Molecular Plant-Microbe Interactions, 27 (9), 956-964, https://dx.doi.org/10.1094/MPMI-03-14-0083-R, https://hal.inrae.fr/hal-02938143
Marta Marchetti, Alain Jauneau, Delphine Capela, Philippe Remigi, Carine Gris, Jacques Batut, Catherine Masson (2014). Shaping Bacterial Symbiosis With Legumes by Experimental Evolution. Molecular Plant-Microbe Interactions, 27 (9), 956 - 964, https://dx.doi.org/10.1094/MPMI-03-14-0083-R, https://hal.inrae.fr/hal-02637300
Marta Marchetti, Delphine Capela, Renaud Poincloux, Nacer Benmeradi, Marie-Christine Auriac, Aurélie Le Ru, Isabelle Maridonneau-Parini, Jacques Batut, Catherine Masson (2013). Queuosine biosynthesis is required for sinorhizobium meliloti-induced cytoskeletal modifications on HeLa Cells and symbiosis with . PLoS ONE, 8 (2), e56043, https://dx.doi.org/10.1371/journal.pone.0056043, https://hal.inrae.fr/hal-02650924
Marta Marchetti, Olivier Catrice, Jacques Batut, Catherine Masson (2011). Cupriavidus taiwanensis Bacteroids in Mimosa pudica Indeterminate Nodules Are Not Terminally Differentiated. Applied and Environmental Microbiology, 77 (6), 2161-2164, https://dx.doi.org/10.1128/AEM.02358-10, https://hal.inrae.fr/hal-02644027
Marta Marchetti, Delphine Capela, Michelle Glew, Stéphane Cruveiller, Béatrice Chane-Woon-Ming, Carine Gris, Ton Timmers, Véréna Poinsot, Beatriz-Luz Gilbert, Philipp Heeb, Claudine Medigue, Jacques Batut, Catherine Masson-Boivin (2010). Experimental Evolution of a Plant Pathogen into a Legume Symbiont. PLoS Biology, 8 (1), e1000280, https://dx.doi.org/10.1371/journal.pbio.1000280, https://hal.inrae.fr/hal-02665813
Julie Claudinon, Pauline Gonnord, Emilie Beslard, Marta Marchetti, Keith Mitchell, Cédric Boularan, Ludger Johannes, Pierre Eid, Christophe Lamaze (2009). Palmitoylation of interferon-alpha (IFN-alpha) receptor subunit IFNAR1 is required for the activation of Stat1 and Stat2 by IFN-alpha.. Journal of Biological Chemistry, 284 (36), 24328-40, https://dx.doi.org/10.1074/jbc.M109.021915, https://hal.inrae.fr/hal-02655804
Claire Amadou, Géraldine Pascal, Sophie Mangenot, Michelle Glew, Cyril Bontemps, Delphine Capela, Sébastien Carrere, Stéphane Cruveiller, Carole Dossat, Aurélie Lajus, Marta Marchetti, Véréna Poinsot, Zoé Rouy, Bertrand Servin, Maged Saad, Chantal Schenowitz, Valérie Barbe, Jacques Batut, Claudine Médigue, Catherine Masson-Boivin (2008-07-30). Genome sequence of the -rhizobium Cupriavidus taiwanensis and comparative genomics of rhizobia. Genome Research, 18 (9), 1472 - 1483, https://dx.doi.org/10.1101/gr.076448.108, https://hal.univ-lorraine.fr/hal-01655676
Peggy Robinet, Alexandre Fradagrada, Marie-Noelle Monier, Marta Marchetti, Anne Cogny, Nicole Moatti, Jean-Louis Paul, Benoît Vedie, Christophe Lamaze (2006). Dynamin is involved in endolysosomal cholesterol delivery to the endoplasmic reticulum: role in cholesterol homeostasis.. Traffic, 7 (7), 811-23, https://dx.doi.org/10.1111/j.1600-0854.2006.00435.x, https://hal.inrae.fr/hal-02657034
Marta Marchetti, Marie-Noelle Monier, Alexandre Fradagrada, Keith Mitchell, Florence Baychelier, Pierre Eid, Ludger Johannes, Christophe Lamaze (2006). Stat-mediated signaling induced by type I and type II interferons (IFNs) is differentially controlled through lipid microdomain association and clathrin-dependent endocytosis of IFN receptors.. Molecular Biology of the Cell, 17 (7), 2896-909, https://dx.doi.org/10.1091/mbc.e06-01-0076, https://hal.inrae.fr/hal-02656874
Marta Marchetti, Jean Claude Sirard, Philippe Sansonetti, Eric Pringault, Sophie Kerneis (2004). Interaction of pathogenic bacteria with rabbit appendix M cells: bacterial motility is a key feature in vivo. Microbes and Infection, 6 (6), 521-528, https://dx.doi.org/10.1016/j.micinf.2004.02.009, https://hal.inrae.fr/hal-02675770
Marta Marchetti, Elise Caliot, Eric Pringault (2003). Chronic acid exposure leads to activation of the cdx2 intestinal homeobox gene in a long-term culture of mouse esophageal keratinocytes.. Journal of Cell Science, 116 (Pt 8), 1429-36, https://hal.inrae.fr/hal-02676150
Marta Marchetti, Rino Rappuoli (2002). Isogenic mutants of the cag pathogenicity island of Helicobacter pylori in the mouse model of infection: effects on colonization efficiency.. Microbiology, 148 (Pt 5), 1447-56, https://dx.doi.org/10.1099/00221287-148-5-1447, https://hal.inrae.fr/hal-02675774
Marta Marchetti, M Rossi, V Giannelli, M M Giuliani, M Pizza, S Censini, A Covacci, P Massari, C Pagliaccia, R Manetti, J L Telford, G Douce, G Dougan, R Rappuoli, P Ghiara (1998). Protection against Helicobacter pylori infection in mice by intragastric vaccination with H. pylori antigens is achieved using a non-toxic mutant of E. coli heat-labile enterotoxin (LT) as adjuvant.. Vaccine, 16 (1), 33-7, https://hal.inrae.fr/hal-02693337
P Ghiara, M Rossi, Marta Marchetti, A Di Tommaso, C Vindigni, F Ciampolini, A Covacci, J L Telford, M T de Magistris, M Pizza, R Rappuoli, G del Giudice (1997). Therapeutic intragastric vaccination against Helicobacter pylori in mice eradicates an otherwise chronic infection and confers protection against reinfection.. Infection and Immunity, 65 (12), 4996-5002, https://hal.inrae.fr/hal-02687597
R Manetti, P Massari, Marta Marchetti, C Magagnoli, S Nuti, P Lupetti, P Ghiara, R Rappuoli, J L Telford (1997). Detoxification of the Helicobacter pylori cytotoxin.. Infection and Immunity, 65 (11), 4615-9, https://hal.inrae.fr/hal-02692196
Marta Marchetti, B Aricò, D Burroni, N Figura, R Rappuoli, P Ghiara (1995). Development of a mouse model of Helicobacter pylori infection that mimics human disease.. Science, 267 (5204), 1655-8, https://hal.inrae.fr/hal-02707700
P Ghiara, Marta Marchetti, M J Blaser, M K Tummuru, T L Cover, E D Segal, L S Tompkins, R Rappuoli (1995). Role of the Helicobacter pylori virulence factors vacuolating cytotoxin, CagA, and urease in a mouse model of disease.. Infection and Immunity, 63 (10), 4154-60, https://hal.inrae.fr/hal-02712048
LIPME - Laboratoire des interactions plantes-microbes-environnement
UMR CNRS-INRA 2594/441
24, chemin de borderouge- Auzeville
CS 52627
31326 Castanet-Tolosan Cedex
France
Valérie Pacquit
Senior Lecturer UT3-Paul Sabatier
RESEARCH SUBJECTS
My research interest is to decipher the molecular mechanisms involved in plant adaptation in response to environmental signals.
I completed my PhD in the Institut de Biotechnologie des Plantes (IBP) at Paris XI University under the supervision of Prof P. Gadal and Dr J. Vidal, in 1995. My thesis project was focused on the photoregulation of the Phosphoenolpyruvate carboxylase (PEPC) implicated in C4 photosynthesis, more particularly on the identification of the PEPC-kinase and the components of the signal transduction pathway. During my post-doctoral contract in Prof. G. Hardie’s group in the MRC-PPU at the University of Dundee (Scotland), the aim of my research was to define the regulation by phosphorylation of the plant homologous of the mamalian AMP-activated protein kinase (APMK) and the yeast SNF1. In october 1996, I was appointed lecturer of the University of Toulouse3, Paul Sabatier. My research works successively dealt with the functional study of AtLecRK-a genes encoding Receptor-like Kinases and the plant cell wall dynamics with a focus on (i) the transcriptional regulation of genes involved in lignin biosynthesis in Eucalyptus (ii) the fonction of cell wall proteins and especially AtLTP2, a Lipid Transfer Protein implicated in the cuticule-cell wall integrity.
Since september 2020, I have joined the Plant Resistance Pathways Dynamics and Adaptation to Climate Change, REACH team in LIPME, to expand research of the identification of mechanisms involved in plant immunity against pathogenic bacteria and the characterization of its signalling pathway.
Despite major advances in our understanding in NLRs activation, there is little information relating to the composition and the dynamics of proximal proteome that involves in activation and in cell signalling of NLRs. Based on previous 2-hybrid screening, selected Arabidopsis thaliana proteins found to potentially interact with the PopP2 Ralstonia solanacearum effector, will be functionnaly characterized. Moreover, the recent proximity labeling technology is carried out for inventorying proteome which in vivo interacts with PopP2 and its associated NLRs immune receptors. The aim is to establish the most exhaustive list of PopP2 protein partners, to define their molecular mechanism of action and their function in the context of virulence or avirulence interactions mediated by PopP2.
TRAINING
Academic training : I did my graduate studies at the University of Paris XI (Orsay Faculty of Sciences) :
-Doctoral thesis (Honorable mention and congratulations of the jury)
-DEA in Molecular and Cellular Plant Biology (with honors)
EDUCATION
Teaching :
At the University Toulouse3-Paul Sabatier, I teach the disciplines of Molecular Genetics, Cellular and Molecular Biology, Biochemistry and Biotechnology and Plant Molecular Physiology at the Licence and Master levels.
My pedagogical (co-) responsibilities (Training and Teaching Units) :
-Master Biotechnologies "Quality and Safety of Health Products and Food" specialty and "Bio-Engineering, Research and Biomedical Application" specialty (from 2022)
-Master Bioengineering - Plant Biotechnologies (2000-2016)
-IUP Bioengineering - Plant Biotechnologies (1997-2011)
-10 Teaching units of Master 1 and 2 (period 2000 to 2022)
LIPME - Laboratoire des interactions plantes-microbes-environnement
UMR CNRS-INRA 2594/441
24, chemin de borderouge- Auzeville
CS 52627
31326 Castanet-Tolosan Cedex
France
PopP2 effector
Plant–pathogen interactions
Epigenetics & chromatin remodeling
Genome editing (CRISPR-Cas9)
ChIP-seq & Cut&Runtop
Raphael Culerrier
IEHC CNRS
RESEARCH SUBJECTS
Since joining LIPME, I have focused (i) on finalizing work related to the manipulation of two Arabidopsis epigenetic readers by the PopP2 effector (GTE9 and GTE11), and (ii) on developing new tools to identify chromatin regions targeted by this effector (ChIP-seq, Cut&Run).
(i) To validate the importance of GTE9 and GTE11 targeting by PopP2 in Ralstonia pathogenicity, I was entrusted with a loss-of-function approach. This involves generating gte9 and gte11 null mutants in the susceptible Col-0 accession using CRISPR-Cas9 genome editing, and determining whether their phenotypic response to Ralstonia differs from that of the wild type.
(ii) The team has recently obtained data suggesting that PopP2 may target various chromatin-remodeling complexes, similar to effectors produced by animal pathogenic bacteria. To investigate the impact of PopP2 on the A. thaliana epigenome, my project aims to develop ChIP-seq and Cut&Run approaches to precisely identify loci whose transcriptional regulation is directly affected by PopP2 (in collaboration with the Chromatin Dynamics team, IPS2).
TRAINING
After enrolling at ESTBA (École Supérieure des Techniques de Biologie Appliquée) to complete a BTS in Biological Analysis, I had the opportunity to continue my studies at the same institution thanks to the then-innovative creation of professional bachelor’s degrees. I joined the “Immunology and Research” professional bachelor’s program, which enabled me to spend a year working in a CNRS laboratory.
Following this experience, I successfully passed the competitive examination for Assistant Engineer and have since worked in several laboratories within well-recognized research units, covering a variety of scientific fields:
· Cancer Research: study of the genetic disease Xeroderma Pigmentosum
(1997–2004: Assistant Engineer – UPR 2169 – Institut Gustave Roussy – Villejuif)
· Immunology: identification and characterization of plant epitopes responsible for cross-reactive allergies in children
(2004–2010: Assistant Engineer – UMR 5546 – LRSV – Auzeville)
· Cell Cycle: study of cellular senescence mechanisms, with a strong focus on epigenetics—a field that has fascinated me ever since
(2010–2021: Research Engineer – UMR 5088 – CBI – Toulouse)
Since 2022, I have held the position of Senior Research Engineer at UMR 2594 – LIPME – Auzeville.
SCIENTIFIC PRODUCTION
SCIENCE AND SOCIETY
I have served several times as a DECLICS ambassador, responsible for promoting scientific research and sharing our work with high school students in their final year of the science track
LIPME - Laboratoire des interactions plantes-microbes-environnement
UMR CNRS-INRA 2594/441
24, chemin de borderouge- Auzeville
CS 52627
31326 Castanet-Tolosan Cedex
France
Sarah Carpentier
PhD student
RESEARCH SUBJECTS
Recent funding
TRAINING
Recent funding
RECENT FUNDING
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LIPME - Laboratoire des interactions plantes-microbes-environnement
UMR CNRS-INRA 2594/441
24, chemin de borderouge- Auzeville
CS 52627
31326 Castanet-Tolosan Cedex
France
Key words
Bodelot Antoine
IR CDD
RESEARCH SUBJECTS
My research topics focus on the study of plant responses to biotic and abiotic stresses. In the ELEVATION project (Exploiting thermostabLE NLR VAriants to mainTain plant disease resIstance in the cOntext of climate warming) in collaboration with iMean company, we try to develop a prediction model which will alow us to predict the thermostability of NLR (NOD-Like Receptors) resistance genes. To develop this model, we use the REACH Team model genes, RPS4/RRS1, which confer a resistance to Ralstonia solanacearum.
TRAINING
After getting a master level from a competitive agronomic school (Instiut Agro, Rennes-Angers center) majoring in Plant Protection and Environment in 2020, I did a thesis in the ResPom team localized in the Institut de Recherche en Horticulture et Semences (IRHS) in Angers. My thesis project was supervised by Marie-Noëlle Brisset, Emilie Vergne and Alexandre Degrave and dealt with on the functional characterization of an apple multigenic genes family called Malus domestica agglutinins (MdAGGs) in the resistance against the phytopathogenic bacteria Erwinia amylovora, the causal agent of fire blight. I used several genomic approaches such as the gene editing of the coding sequences of these protein through the CRISPR/Cas9 system or the constitutive overexpression of one of these proteins. Then, these different apple lines were characterized at the molecular, biochemical and phenotypical level in E. amylovora infection conditions. I also used an intragenesis approach to get apple intragenic lines resistant to fire blight.
SCIENTIFIC PRODUCTION
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LIPME - Laboratoire des interactions plantes-microbes-environnement
UMR CNRS-INRA 2594/441
24, chemin de borderouge- Auzeville
CS 52627
31326 Castanet-Tolosan Cedex
France
Key words
Maxime Girard
PhD student
RESEARCH SUBJECTS
TRAINING
Recent funding
RECENT FUNDING
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