Transgenic plant production

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• We perform routine transformations and regeneration of stable transgenic for Arabidopsis thaliana and Medicago truncatula, via Agrobacterium tumefaciens.

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• for Medicago truncatula, the stable transgenic lines are obtained via a regeneration via embryogenesis (Chabaud et al. 1996) 

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• For Arabidopsis thaliana transformation we use the the "floral dip" method (Bechtold et al. 1993)

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• Tomato is transformed with Argrobacterium tumefaciens starting with cotyledons.

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• For Brachypodium distachyon, the model monocotyledon plant, the transformation technique was transferred from our colleagues at INRAE Versailles. It consist in the Agrobacterium tumefaciens mediated transformation via somatic embryogenesis.

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In developement

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• In collaboration with a team at LIPME, the service developed a quick method for the transient expression of Medicago truncatula roots (Rembliere et al. 2018).

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• The service has developed Medicago truncatula mutant lines that are more embryogenic in order to increase the transformation efficiency.

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trainees

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