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Mots clés
Tournesol, génétique, génomique, phénotypage, sécheresse, froid, chaud, hautes températures, Heliaphen, Polliphen, Phenotoul
Nicolas Langlade
Fonction
Chef d'équipe ASTR
Directeur de recherche 2
SUJETS DE RECHERCHE
I conduct my multi-disciplinary research in close collaboration with private partners such as breeding companies, technical institute Terres Inovia and different academic labs covering agronomy, systems biology, and evolution.
My aim is to understand the genetic and molecular control of sunflower response to its abiotic environment and to provide knowledge for the adaptation of sunflower to agroecological agrosystems.
This includes:
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Identifying genomic regions and genetic polymorphisms controlling plasticity of yield for drought or cold stresses. For this, I collaborate with agronomists (P. Casadebaig and Ph. Debaeke from UMR INRAE AGIR) to model crop functioning and characterize stress scenarios. Using quantitative genetics, this allows us to predict plasticity and identify QTL or clone the genes involved in yield tolerance.
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Identify the molecular response of sunflower to drought stress at different omics levels: infer gene regulatory network, integrate proteomics, metabolomics, transcriptomics and physiological data in a systems biology approach
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Identify the sunflower ideotype adapted to novel abiotic stress scenarios induced by the agroecological transition (relay-cropping, inter-cropping) at the phenotypic, molecular and genetic levels
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Identify the impact of climate change on sunflower pollinator attractivity and its molecular and genetic control
After a strong involvement in sunflower genomics, I believe the next frontier is sunflower phenomics. I participate actively to the high-throughput phenotyping infrastructure Phenotoul [1] which, since 2019, brings together the TPMP platforms under controlled conditions, Heliaphen under semi-controlled conditions and Agrophen in the field.
In addition, I am strongly committed to the animation of the French sunflower research community through the coordination of the SUNRISE Investment for the Future Project, the animation of the sunflower technical commission of Promosol, the participation in the activities of the variety registration institution CTPS (in the Sunflower-Soy section and in the VATE). Internationally, I participate to the board of the International Sunflower Association (ISA) and on the scientific committee of the ICSG (International Consortium for Sunflower Genomics).
FORMATION
I started my career in Switzerland (PhD at the University of Neuchâtel, 1998-2002) with Enrico Martinoia on the physiological and molecular study of organic acid exudation and development of cluster roots in lupin.
Then I worked in the United-Kingdom (John Innes Center, Norwich, 2002-2006), with Enrico Coen, focusing on the evolutionary development of leaves and flower colour in Antirrhinum.
Recruited in 2006 at INRA as a post-doctorate researcher at the Plant Reproduction and Development Laboratory (Ecole Normale Supérieure de Lyon) with Peter Rogowsky, I briefly worked on the development of corn kernels.
I joined LIPME (INRAE Occitanie Toulouse) as senior researcher in 2007 and I am now research director since 2016. I lead the ASTR research team (Abiotic Stress Tolerance in sunfloweR) which aims at identifying the genetic and molecular bases of adaptation of sunflower to abiotic constraints (drought and cold) at physiological and evolutionary levels.
I obtained my Habilitation à Diriger les Recherches on the integration of environmental and evolutionary constraints in gene regulatory networks, in 2015 from the University of Toulouse.
FINANCEMENTS RECENTS
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Projet Investissment d’Avenir Sunrise[1] , coordinateur, responsable des WP management, phénotypage, et génétique, 2012-2020
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Projet bilateral INRAE Syngenta COPER, coordinateur, 2020-2025
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Projet Promosol Heliopollen, coordinateur, 2021-2023
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Projet Plant2Pro CIMS-ON, responsable WP Phénotypage, 2020-2023
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Projet Investissement d’Avenir Phenome-Emphasis[2] , partenaire, 2012-2024
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Projet CASDAR IPHARD, responsable de WP Phénotypage, 2019-2022
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Projet Promosol et semenciers Heliawild, coordinateur, 2022-2025
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Projet Promosol Heliasen, coordinateur, 2018-2020
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Projet bilateral INRAE Terres Inovia EPhECTS, coordinateur, 2018-2020
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Projet Promosol Heliadiv, partenaire, 2015-2018
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Projet Promosol Heliadiv 2, partenaire 2018-2020
COLLABORATIONS
At LIPME
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CRB Tournesol (tous les projets)
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Plateforme bioinformatique (SUNRISE, ICSG, COPER, Heliopollen)
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Equipe Sunflower Pest Interaction (SUNRISE, ICSG, CIMS-ON)
At INRAE
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AGIR (SUNRISE, CIMS-ON, IPHARD)
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UE APC (CIMS-ON, IPHARD, PhASTER)
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MIAT (SUNRISE)
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BFP (SUNRISE)
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GQE (SUNRISE)
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CNRGV (SUNRISE, ICSG)
At national level
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Sorbonne Université (SUNRISE)
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Terres Inovia (SUNRISE, EPhECTS)
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Syngenta (SUNRISE, ICSG, COPER)
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Lidea, MAS, Soltis, RAGT, Innolea (SUNRISE, ICSG, Heliawild)
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Corteva (ICSG, Heliawild)
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KWS (ICSG)
At international level
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INTA Castelar Buenos Aires AR (HeliaSen)
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U. British Columbia Vancouver (ICSG)
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U. Georgia Athens (ICSG)
ENSEIGNEMENT
I present my research and phenotyping platforms at the university of Toulouse at the Master TULIP-Graduate School [1] and at the University of Cordoba (Argentina) at the Master level.
[1]https://www.labex-tulip.fr/La-Graduate-School/Le-Master-TULIP-GS
MEMBRE COMITE
I participate to several organizations :
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Board member of the International Sunflower Association
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Member of the scientific committee of the International Consortium for Sunflower Genomics
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Animator of the Promosol sunflower committee
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Member of the Promosol and FSRSO scientific committee
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Member of the CTPS “Sunflower, soy and castorbean” section and sunflower VATE commission
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Member of the UE Agroecologie et Phenotypage des Cultures user committee
PRODUCTION SCIENTIFIQUE
Productions scientifiques sur le tournesol
Production scientifique lors de mes positions précédentes
Costa, M.M.R., Yang, S., Critchley, J., Feng, X., Wilson, Y., Langlade, N., Copsey, L., Hudson, A., 2012. The genetic basis for natural variation in heteroblasty in Antirrhinum. New Phytologist 196, 1251–1259.
Dubois, A., Raymond, O., Maene, M., Baudino, S., Langlade, N.B., Boltz, V., Vergne, P., Bendahmane, M., 2010. Tinkering with the C-function: a molecular frame for the selection of double flowers in cultivated roses. PLoS ONE 5, e9288.
Bensmihen, S., Hanna, A.I., Langlade, N.B., Micol, J.L., Bangham, A., Coen, E.S., 2008. Mutational spaces for leaf shape and size. HFSP journal 2, 110–120.
Whibley, A.C*., Langlade, N.B.*, Andalo, C., Hanna, A.I., Bangham, A., Thébaud, C., Coen, E., 2006. Evolutionary paths underlying flower color variation in Antirrhinum. Science 313, 963–966. *co-first authors
Weisskopf, L., Tomasi, N., Santelia, D., Martinoia, E., Langlade, N.B., Tabacchi, R., Abou- Mansour, E., 2006. Isoflavonoid exudation from white lupin roots is influenced by phosphate supply, root type and cluster-root stage. New Phytologist 171, 657–668.
Dreyer, C., Langlade, N., Hoffmann, M., Tripathi, N., Russell, S., Weigel, D., 2006. Inheritance And Natural Variation Of Adult Male Ornaments Of The Guppy: Pp010p. Reticulata: Pp010. Pigment Cell Research 19, 519.
Langlade, N.B., Feng, X., Dransfield, T., Copsey, L., Hanna, A.I., Thébaud, C., Bangham, A., Hudson, A., Coen, E., 2005. Evolution through genetically controlled allometry space. Proceedings of the National Academy of Sciences of the united states of america 102, 10221–10226.
Kania, A.*, Langlade, N.*, Martinoia, E., Neumann, G., 2003. Phosphorus deficiency- induced modifications in citrate catabolism and in cytosolic pH as related to citrate exudation in cluster roots of white lupin. Plant and soil 248, 117–127. *co-first authors
Langlade, N., Messerli, G., Weisskopf, L., Plaza, S., Tomasi, N., Smutny, J., Neumann, G., Martinoia, E., Massonneau, A., 2002. ATP citrate lyase: cloning, heterologous expression and possible implication in root organic acid metabolism and excretion. Plant, Cell & Environment 25, 1561–1569.
Massonneau, A., Langlade, N., Léon, S., Smutny, J., Vogt, E., Neumann, G., Martinoia, E., 2001. Metabolic changes associated with cluster root development in white lupin (Lupinus albus L.): relationship between organic acid excretion, sucrose metabolism and energy status. Planta 213, 534–542.
Neumann, G., Massonneau, A., Langlade, N., Dinkelaker, B., Hengeler, C., Römheld, V., Martinoia, E., 2000. Physiological Aspects of Cluster Root Function and Development in Phosphorus-deficient White Lupin ( Lupinus albus L.). Annals of Botany 85, 909–919. https://doi.org/10.1006/anbo.2000.1135
LIENS
HOBBY
Cooking, sculpting, sport
Adresse
Tel
Mots clés
Olivier Catrice
Fonction
Assistant ingénieur
SUJETS DE RECHERCHE
Tolérance aux stress abiotiques
Interactions Tournesols-Pollinisateurs-Environnement
FORMATION
Master Biologie Intégrative et Physiologie Végétales Paris 6
FINANCEMENTS RECENTS
HelioPollen : Financement 38 k€ par Promosol
COLLABORATIONS
Nicky Creux, Plant and Soil Sciences, Forestry & Agricultural Biotechnology Institute, Pretoria, République d’Afrique du Sud (2021)
MEMBRE COMITE
Membre du comité Développement Durable du LIPME
Représentant CGT au CHSCT du Centre Occitanie-Toulouse
PRODUCTION SCIENTIFIQUE
The genome sequence of the grape phylloxera provides insights into the evolution, adaptation, and invasion routes of an iconic pest, BMC Biol. 2020; 18: 90. doi: 10.1186/s12915-020-00820-5 ; Correction in: BMC Biol. 2020; 18: 123.
Jejunal villus absorption and paracellular tight junction permeability are major routes for early intestinal uptake of food-grade TiO2 particles: an in vivo and ex vivo study in mice Part Fibre Toxicol. 2020; 17: 26. doi: 10.1186/s12989-020-00357-z
The sunflower genome provides insights into oil metabolism, flowering and Asterid evolution. Nature. 2017 Jun 1;546(7656):148-152. doi: 10.1038/nature22380.
Plant Physiol. 2016 Jul;171(3):2256-76. doi: 10.1104/pp.16.00711.
Unraveling the early molecular and physiological mechanisms involved in response to phenanthrene exposure BMC Genomics. 2016; 17: 818. doi: 10.1186/s12864-016-3133-0
The CCAAT box-binding transcription factor NF-YA1 controls rhizobial infection. J Exp Bot. 2014 Feb;65(2):481-94. doi: 10.1093/jxb/ert392..
HOBBY
Apiculture
Arboriculture
Musique et lutherie
Voile et construction navale
Adresse
Tel
Mots clés
Nicolas Pouilly
Fonction
Assistant ingénieur
SUJETS DE RECHERCHE
Tolérance aux stress abiotiques
FORMATION
Maîtrise de Biochimie, Université de Pau et des Pays de l’Adour
COLLABORATIONS
Hélène Badouin, Laboratoire de Biométrie et Biologie Évolutive (Université Lyon1/CNRS)
PRODUCTION SCIENTIFIQUE
- Calderón-González, Á., Pouilly, N., Muños, S., Grand, X., Coque, M., Velasco, L., & Pérez-Vich, B. (2019). A SSR-SNP linkage map of the parasitic weed Orobanche cumana Wallr. including a gene for plant pigmentation. Frontiers in Plant Science, 10, 797.
- Bonnafous, F., Fievet, G., Blanchet, N., Boniface, M.C., Carrère, S., Gouzy, J., Legrand, L., Marage, G., Bret-Mestries, E., Munos, S., Pouilly, N., Vincourt, P., Langlade, N.B., & Mangin, B. (2018). Comparison of GWAS models to identify non-additive genetic control of flowering time in sunflower hybrids. Theoretical and Applied Genetics, 131(2), 319-332.
- Bordat, A., Marchand, G., Langlade, N.B., Pouilly, N., Muños, S., Dechamp-Guillaume, G., Vincourt, P. & Bret-Mestries E. (2017). Different genetic architectures underlie crop responses to the same pathogen: the {Helianthus annuus* Phoma macdonaldii} interaction case for black stem disease and premature ripening. BMC plant biology, 17(1), 167.
- Mangin, B., Bonnafous, F., Blanchet, N., Boniface, M.C., Bret-Mestries, E, Carrère, S., Cottret, L., Legrand, L., Marage, G., Pegot-Espagnet, P., Munos, S., Pouilly, N., Vear, F., Vincourt, P., & Langlade, N.B. (2017). Genomic Prediction of Sunflower Hybrids Oil Content. Frontiers in plant science, 8, 1633.
- Mangin, B., Casadebaig, P., Cadic, E., Blanchet, N., Boniface, M.C., Carrère, S., Gouzy, J., Legrand, L., Mayjonade, B., Pouilly, N., André, T., Coque, M., Piquemal, J., Laporte, M., Vincourt, P., Muños, S., & Langlade, N.B. (2017). Genetic control of plasticity of oil yield for combined abiotic stresses using a joint approach of crop modelling and genome-wide association. Plant, Cell and Environment 40(10):2276-2291.
- Badouin, H., Gouzy, J., Grassa, CJ., Murat, F., Staton, SE., Cottret, L., Lelandais-Brière, C., Owens, G., Carrère, S., Mayjonade, B., et al. (2017). The sunflower genome illuminates the evolutionary history of the Asterids and provides new insights into oil metabolism and flowering time. Nature 546, 148–152.
- Mangin, B., Pouilly, N., Boniface, M.C., Langlade, N.B., Vincourt, P., Vear, F. & Muños, S. (2017). Molecular diversity of sunflower populations maintained as genetic resources is affected by multiplication processes and breeding for major traits. Theoretical and Applied Genetics 130(6):1099-1112.
- Mayjonade, B., Gouzy, J., Donnadieu, C., Pouilly, N., Marande, W., Callot, C., Langlade, NB., & Muños, S. (2016). Extraction of high-molecular-weight genomic DNA for the long-read sequencing of single molecules. BioTechniques 61: 203–205.
- Louarn, J., Boniface, M.C., Pouilly, N., Velasco, L., Pérez-Vich, B., Vincourt, P., & Muños, S. (2016). Sunflower Resistance to Broomrape (Orobanche cumana) Is Controlled by Specific QTLs for Different Parasitism Stages. Front Plant Sciences 7: 590.
- Gascuel, Q., Bordat, A., Sallet, E., Pouilly, N., Carrère, S., Roux, F., Vincourt, P., & Godiard, L. (2016). Effector Polymorphisms of the Sunflower Downy Mildew Pathogen Plasmopara halstedii and Their Use to Identify Pathotypes from Field Isolates. PloS one, 11(2), e0148513.
- Cadic, E., Coque, M., Vear, F., Grezes-Besset, B., Pauquet, J., Piquemal, J., Lippi Y., Blanchard, P., Romestant, M., Pouilly, N., Rengel, D., Gouzy, J., Langlade, N.B., Mangin, B., & Vincourt, P. (2013). Combined linkage and association mapping of flowering time in Sunflower (Helianthus annuus L.). Theoretical and Applied Genetics 126:1337-1356.
- Vincourt, P., As-Sadi, F., Bordat, A., Langlade, N.B., Gouzy, J., Pouilly, N., Lippi, Y., Serre, F., Godiard, L., Tourvieille de Labrouhe, D., & Vear, F. (2012). Consensus mapping of major resistance genes and independent QTL for quantitative resistance to sunflower downy mildew. Theoretical and Applied Genetics 125:909-920.
- Merah, O., Langlade, N.B., Alignan, M., Roche, J., Pouilly, N., Lippi, Y., Vear, F., Cerny, M., Bouniols, A., Mouloungui, Z., & Vincourt, P. (2012). Genetic analysis of phytosterol content in sunflower seeds. Theoretical and Applied Genetics 125:1589-1601.
- Delourme, R., Piel, N., Horvais, R., Pouilly, N., Domin, C., Vallée, P., Falentin, C., Manzanares-Dauleux, M., & Renard, M. (2008). Molecular and phenotypic characterization of near isogenic lines at QTL for quantitative resistance to Leptosphaeria maculans in oilseed rape (Brassica napus L.). Theoretical and Applied Genetics 117(7): 1055-1067.
- Pouilly, N., Delourme, R., Alix, K., & Jenczewski, E. (2008). Repetitive sequence-derived markers tag centromeres and telomeres and provide insights into chromosome evolution in Brassica napus. Chromosome Research 16(5): 683-700.
- Prieto, J.L., Pouilly, N., Jenczewski, E., Deragon, JM., & Chêvre, A.M. (2005). Development of crop-specific transposable element (SINE) markers for studying gene flow from oilseed rape to wild radish. Theoretical and Applied Genetics 111(3): 446-455.
Adresse
Tel
Mots clés
Marco Moroldo
Fonction
Ingénieur de recherche
SUJETS DE RECHERCHE
Analyse de données omiques, intégration de données, génétique d’association
FORMATION
Financements récents
FINANCEMENTS RECENTS
J’ai démarré ma carrière en Italie faisant un doctorat à l’université de Udine, dans l’équipe de Michele Morgante, ensuite je suis parti pour un post-doc en France à l’INRAE, en travaillant sur la cartographie physique et sur la diversité nucléotidique des gènes de résistance de la vigne.
J’ai été recruté à l’INRAE en 2010 en tant que responsable de la plate-forme de génomique @BRIDGe (département génétique animale), qui proposait plusieurs services dans le secteur de la génomique, notamment des analyses de transcriptome par puce et du reséquençage NGS par capture de séquence.
Ensuite, entre 2016 et 2020 j’ai travaillé en tant qu’analyste de données au sein de l’équipe PSGEN (département génétique animale). Dans ce contexte, je me suis occupé surtout de données de transcriptome et d’intégration entre données de transcriptome et d’autres données omiques.
J’ai rejoint l’équipe ASTR en 2021 pour m’occuper d’analyse de données omiques, de génétique d’association et pour développer à moyen terme ma propre thématique de recherche.
COLLABORATIONS
Collaborations
ENSEIGNEMENT
Enseignements
MEMBRE COMITE
Membre comité
ACTIVITE EDITORIALE
Activités éditoriales
PRODUCTION SCIENTIFIQUE
Google Citations (https://scholar.google.ca/citations?user=gGoR5tkAAAAJ&hl=en)
LIENS
Jean Leconte
Fonction
Post-doctoral researcher
SUJETS DE RECHERCHE
J'ai entamé ma recherche post-doctorale en étudiant le métabolome du tournesol en réponse à la sécheresse et aux hautes températures. Je m'interesse notamment aux potentielles modifications du métabolome du nectar de tournesol induites par la sécheresse et les hautes températures.
Je travaille également au développement d'une méthode haut-débit de mesure de la qualité des graines en utilisant la technique des NIR (Near Infra-Red).
Durant ma thèse (2021-2024), j'ai étudié le contrôle génétique de la plasticité de trait physio-morphologiques et de rendement chez le tournesol exposé au stress froid non gélif (chilling) en début de cycle. Pour cela j'ai effectué un phénotypage haut débit du développement du tournesol à différents stades de développement. J'ai cultivé des tournesol dans des chambres de culture en condition contrôlées, en condition semi-contrôlées sur la plateforme Heliaphen et au champ. Ces données ont été intégré à un travaille de cartographie fine d'un QTL de tolérance au froid dans le but d'identifier des gènes et fonctions candidates.
Mots clés
FORMATION
Licence de bioinformatique, Université de Paris (Anciennement Paris Diderot, Paris 7)
Licence en biologie des organismes, des populations et de l'environnement (Université Paul Sabatier)
Master Adaptation, Developpement et Amélioration des plantes en interaction avec des Microorganismes (Université Paul Sabatier)
PhD on the genetic control of sunflower plasticity in response to cold stress (Paul Sabatier University)
FINANCEMENTS RECENTS
HELEX Project
PRODUCTION SCIENTIFIQUE
Leconte JML, Marco M, Nicolas B, et al. Multi-scale characterisation of cold response reveals immediate and long-term impacts on cell physiology up to seed composition in sunflower. Plant, Cell & Environment 1-19. https://doi.org/10.1111/pce.14941
Adresse
Tel
Mots clés
Blanchet Nicolas
Fonction
Technicien de recherche
SUJETS DE RECHERCHE
Je suis technicien en expérimentation et production végétale au LIPMe depuis 2011. Je participe à l’étude des réponses du tournesol aux stress froid et au stress hydrique en conditions contrôlées et sur la plateforme de phénotypage haut débit HELIAPHEN. Par ailleurs je suis responsable technique de cette plateforme.
FORMATION
BTS Productions Horticoles
Licence professionnelle Expertise agro-environnementale et conduite de projet
FINANCEMENTS RECENTS
Financements récents
COLLABORATIONS
Collaborations
ENSEIGNEMENT
Enseignements
MEMBRE COMITE
Membre comité
ACTIVITE EDITORIALE
Activités éditoriales
PRODUCTION SCIENTIFIQUE
Productions scientifiques
LIENS
Science & Société
SCIENCE ET SOCIETE
Science & Société
HOBBY
Hobbies
AUTRES AFFILIATION
Autres affiliations
Mots clés
Guillaume Tueux
Fonction
PhD student
SUJETS DE RECHERCHE
Contrôle génétique et moléculaire du microbiote de nectar de tournesol et son impact sur l’attractivité pour les pollinisateurs
FORMATION
Ecole d’ingénieurs de Purpan (2016-2021)
Coordinateur des projets d’innovation ainsi que du phénotypage pour la pollinisation du tournesol chez Syngenta (2022-2023)
FINANCEMENTS RECENTS
Projet Attracthol financement Carnot Plant2Pro
COLLABORATIONS
Collaboration avec Terres Inovia
LIENS
Mots clés
Stan Chabert
Fonction
Chercheur post-doctoral
SUJETS DE RECHERCHE
Contrôle génétique et moléculaire du microbiote de nectar de tournesol et son impact sur l’attractivité pour les pollinisateurs
FORMATION
Ecole d’ingénieurs de Purpan (2016-2021)
Coordinateur des projets d’innovation ainsi que du phénotypage pour la pollinisation du tournesol chez Syngenta (2022-2023)
FINANCEMENTS RECENTS
Projet Attracthol financement Carnot Plant2Pro
COLLABORATIONS
Collaboration avec Terres Inovia
LIENS