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I'm a paragraph. I'm connected to your collection through a dataset. To update me, go to the Data Manager. The Data Manager is where you store data to use in your site pages, or collect data from site visitors when they submit a form.
This collection in the Data Manager is already set up with some fields and content. To customize it with your own content, you can import a CSV file or simply edit the placeholder text. You can also add more fields which you can connect to other page elements so the content displays on your published site. Remember to sync the collection so your content is live! You can add as many new collections as you need to store or collect data.
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I'm a paragraph. I'm connected to your collection through a dataset. To update me, go to the Data Manager. The Data Manager is where you store data to use in your site pages, or collect data from site visitors when they submit a form.
This collection in the Data Manager is already set up with some fields and content. To customize it with your own content, you can import a CSV file or simply edit the placeholder text. You can also add more fields which you can connect to other page elements so the content displays on your published site. Remember to sync the collection so your content is live! You can add as many new collections as you need to store or collect data.
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I'm a paragraph. I'm connected to your collection through a dataset. To update me, go to the Data Manager. The Data Manager is where you store data to use in your site pages, or collect data from site visitors when they submit a form.
This collection in the Data Manager is already set up with some fields and content. To customize it with your own content, you can import a CSV file or simply edit the placeholder text. You can also add more fields which you can connect to other page elements so the content displays on your published site. Remember to sync the collection so your content is live! You can add as many new collections as you need to store or collect data.
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I'm a paragraph. I'm connected to your collection through a dataset. To update me, go to the Data Manager. The Data Manager is where you store data to use in your site pages, or collect data from site visitors when they submit a form.
This collection in the Data Manager is already set up with some fields and content. To customize it with your own content, you can import a CSV file or simply edit the placeholder text. You can also add more fields which you can connect to other page elements so the content displays on your published site. Remember to sync the collection so your content is live! You can add as many new collections as you need to store or collect data.
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I'm a paragraph. I'm connected to your collection through a dataset. To update me, go to the Data Manager. The Data Manager is where you store data to use in your site pages, or collect data from site visitors when they submit a form.
This collection in the Data Manager is already set up with some fields and content. To customize it with your own content, you can import a CSV file or simply edit the placeholder text. You can also add more fields which you can connect to other page elements so the content displays on your published site. Remember to sync the collection so your content is live! You can add as many new collections as you need to store or collect data.
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I'm a paragraph. I'm connected to your collection through a dataset. To update me, go to the Data Manager. The Data Manager is where you store data to use in your site pages, or collect data from site visitors when they submit a form.
This collection in the Data Manager is already set up with some fields and content. To customize it with your own content, you can import a CSV file or simply edit the placeholder text. You can also add more fields which you can connect to other page elements so the content displays on your published site. Remember to sync the collection so your content is live! You can add as many new collections as you need to store or collect data.
Arabidopsis
Transformation d'Arabidopsis thaliana
METHODES
Activité de production des plantes transgéniques
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Le service assure en routine la transformation et la régénération de plantes transgéniques stables pour les espèces Arabidopsis thaliana et Medicago truncatula via Agrobacterium tumefaciens.
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Pour l'espèce Medicago truncatula, les plantes transgéniques stables sont obtenues via une régénération par embryogenèse (Chabaud et al.,1996).
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La méthode par trempage des inflorescences (Betchold et al., 1993) est appliquée à l'espèce Arabidopsis thaliana
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La tomate (Solanum lycopersicum) pour laquelle un protocole de transformation par Agrobacterium tumefaciens à partir des cotylédons vient d'être mis en place au laboratoire.
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La monocotylédone modèle Brachypodium distachyon pour laquelle le service a bénéficié d’un transfert de technologie de l’INRAE de Versailles et ainsi pu mettre en place la transformation par Agrobacterium tumefaciens via la régénération in vitro par embryogenèse somatique.
Activité de développement
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En collaboration avec une équipe de recherche du LIPME, le service a conçu une méthode de transformation rapide pour la réalisation d’expression transitoire dans des racines de M. truncatula (Rembliere et al., 2018).
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Le service a également développé des lignées mutantes plus embryogènes chez M. truncatula à des fins de transgénèse.
Stagiaires
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2019-2020
Benjamin Militzer : BTS "Bioanalyses et Contrôles" 1ère et 2ème année, Lycée Saliège -Toulouse
"Mise au point d'un protocole de transformation in planta de Medicago truncatula par Agrobacterium tumefaciens"
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2020
Florian Paillas : BTS "Biotechnologies" 1ère année, Lycée Saint Louis-Bordeaux
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PUBLICATIONS
A simple Agrobacterium tumefaciens-mediated transformation method for rapid transgene expression in Medicago truncatula root hairs.
Céline Remblière, Joëlle Fournier, Fernanda De Carvalho-Niebel, Mireille Chabaud. January 2018. Plant Cell Tissue and Organ Culture 132(1) 10.1007/s11240-017-1323-3
Reprogramming of DNA methylation is critical for nodule development in Medicago truncatula.
Satgé C, Moreau S, Sallet E, Lefort G, Auriac MC, Remblière C, Cottret L, Gallardo K, Noirot C, Jardinaud MF, Gamas P. Nat Plants. 2016 Oct 31;2(11):16166. doi: 10.1038/nplants. 2016.166
Vernié T, Camut S, Camps C, Rembliere C, de Carvalho-Niebel F, Mbengue M, Timmers T, Gasciolli V, Thompson R, le Signor C, Lefebvre B, Cullimore J, Hervé C. Plant Physiol. 2016 Apr;170(4):2312-24. doi: 10.1104/pp.15.01694
Lateral root formation and patterning in Medicago truncatula.
Herrbach V, Remblière C, Gough C, Bensmihen S. J Plant Physiol. 2014 Feb 15;171(3-4):301-10. doi: 10.1016/j.jplph.2013.09.006.